Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. We would like to show you a description here but the site won’t allow us. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Add to Cart. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Blank subtraction and standard curve fitting were done automatically by the software. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. microplate reader. Figure 1. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 305 reference level saturation. Cuvette Microplate wells. The dual monochromator optics allow the widest range of. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. 030 fmol Fluorescence Polarization (FP). 6 for further experiment, so I'm confused which i would choose for OD value determination. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. no. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. . 0 out of 5 $ 6,499. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Temperature control. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Fill a clean plate with water. Sensitive fluorescent quantitation of DNA with the Quant-iT. Italic and boldface type are used for emphasis. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Absorbance (optical density, OD) was read at 570 nm and 605. Contact our support team with your questions regarding Molecular Devices products and applications. . The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Endpoint, kinetic, spectrum. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. Our specialization servicing. 380 nm/Exc. The SpectraMax® ABS Plus Microplate Reader can run . A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Use the provided 3. Examples: “Press carefully to engage,” “ Do not. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. microplate reader. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SpectraMax Microplate Readers. Figure 6: Transcreener ADP2 FI assay principle. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Molecular Devices SpectraMax M2E Microplate Reader. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. 76358-624. These lab workhorses are used in drug discovery. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The screw remains inside the instrument. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax Mini User Guide. To resume collecting kinetic measurements within the same file, press the Append button. 304 signal level saturation. 2. An assay plate containing a 15-point standard curve was read on the SpectraMax. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Cell Cytotoxicity assay procedure. com 4. Introduction. We use Spectramax M5 microplate reader in our lab. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Time-. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 2. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. 306 plate air cal fail,low light. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 2. 100 fmol 1536-well (8 µL) 0. The 2-channel configuration is available with 0, 2, or 4 injectors. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Nuclear Receptor. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Multimode Microplate Readers. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. The plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Additionally, user-installable options allow the SpectraMax i3 System to. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0 out of 5 $ 11,999. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Contents of wells were pipetted up and down several times to mix. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Find regional support. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. com. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. 314 low lamp level warning. microplate reader. Molecular Interactions. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. 20 μL of Component A was added to each well of the microplate. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Highly customizable low volume. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Table 1. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. SpectraMax Spectrofluorometers. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 0 out of 5 $ 5,999. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The plate reader optics are tunable for. Published Dec 16, 2020 | Updated May 19, 2023. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. begin reading microplates with seven mouse clicks. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. The drawing shows a standard microplate of 15 mm height covered with a lid. 0 out of 5 $ 23,995 $ 22,495. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 1. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices Spectramax iD3 Microplate Reader. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Instrument settings used with the SpectraMax M5 Microplate Reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). We would like to show you a description here but the site won’t allow us. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Please refer to the Obsolete Microplate Reader Models for more information. AlphaScreen. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Also see:. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Other technologies such as imaging. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The calculated LOQ values were 0. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Table of Contents. begin reading microplates with seven mouse clicks. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Or scan up to 384 wells at any time to determine the best assay conditions. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Other technologies such as imaging. 1. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Molecular Devices. 1. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 1. 0 out of 5 $ 5,999. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. The SpectraMax 190 reader can be integrated with Molecular . Next generation SpectraMax® absorbance microplate readers. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Guides: SpectraMax Mini Installation Guide. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. A preconfigured protocol is available in the software to. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. 303 unable to cal dark current. Selection of Water Constant in PathCheck settings. ELISA Plate Readers / Microplate Readers. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. For more sample throughput, both readersSupplier: Molecular Devices. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Create custom fluorescence assays without having to purchase expensive filter sets. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The plate reader can measure multiple readouts, including UV. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. 1) Select the correct plate and the wells that you want to measure. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax iD5 Multi-Mode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Other features and benefits include: Eliminate changing out filters. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The dual monochromator optics allow. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. . Detailed specifications of each model reader are reported in Section. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Microplate Readers BioTek Epoch 2 Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. With optimized reagents, option to operate the. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. flexibility of monochromator-based systems. SpectraMax Spectrofluorometers. Learn more about its features, specifications, and applications in this user. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Type:Spectrophotometer. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 7°C and the bottom to 36. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 5 12. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. The guide also covers the software features, the specifications, and the accessories of the systems. SpectraMax Microplate Readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. The CellTiter-Glo assay from Promega uses luciferase. SpectraMax M5 reader settings are shown. both cuvette-based and microplate reader applications Microplate Readers. A new technical approach to platelet aggregation studies. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. View the Molecular Devices website. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. 5 12. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. We would like to show you a description here but the site won’t allow us. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. western blot membrane detection in a multimode microplate reader platform. Prerequisites: SoftMax Pro 7. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 2 only: SpectraMax Mini Update. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. More Product Information. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 313 reference gain check fail. This pathlength correction is accomplished only with the use of. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 3) This warranty. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Here, the CVs for each plate were less than 3, and the difference between mean. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. . No accessories are needed and SoftMax® Pro Software. Molecular Devices SpectraMax M2 Multimode Plate Reader. 316 grating motor driver faulty. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. 20 μL of Component A was added to each well of the microplate. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). 306 plate air cal. Or, with the reader specifications noted from the Instrument Description section of your reader's. 470 nm). In addition, the SpectraMax iD5 can be expanded to. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 5 87. Cell Analysis. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. their consistent performance, reliability and durability. Download PDF versions of the SpectraMax Microplate Reader User Guides. For a higher . 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . San Jose, Calif. Maximum signal with lowest background. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. no. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The guide also covers the software features, the specifications, and the accessories of the systems. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 7°C (gradient heating design).